2007, Sedgley, Margaret

'Banksia' species (Plate I) have been cultivated for the international cut flower market for only 20 to 30 years, but there is increasing interest in areas other than the native home, Australia, with production in Israel, South Africa, Hawaii, and California (Ben-Jaacov 1986; Sedgley 1996). Within Australia, 'Banksia' is one of the four most widely planted commercial native genera, but production is based on seedling material and between plant variability is high. 'Banksia' species for the fresh cut flower market must fulfill strict commercial criteria, which include terminal blooms and long stem length (Fig. 1.1), and further research is needed into all aspects of 'Banksia' biology and production. In addition to the fresh cut flower market, 'Banksia' stems are traded as dried and dyed blooms, and a wide range of species is used in environmental horticulture, for the attractive inflorescences and foliage, and to attract birds and other wildlife. Although there has been little work conducted so far on the use of banksias as pot plants, recent developments with related genera suggest that such an approach may be productive (Ben-Jaacov et al. 1989). 'Banksia' wood and cones are turned or incorporated into ornaments, and the timber of some species has been used for furniture.

2006, Glocke, P, Delaporte, K, Collins, G, Sedgley, Margaret

The genus 'Eucalyptus' contains many species suitable for the floriculture and amenity horticulture industries in Australia. A development program has been underway at the University of Adelaide, with the aim of producing new and novel hybrid eucalypt varieties for these industries through controlled pollination between selected ornamental species. As each new hybrid plant reaches reproductive maturity, it is assessed for desirable characters and the very best plants selected for further development, including response to production systems and vegetative propagation. Plants that perform well in cultivation and can be propagated vegetatively will be registered with Plant Breeders Rights and made available through commercial nurseries.

2008, Franks, Tricia K, Yadollahi, Abbas, Wirthensohn, Michelle G, Guerin, Jennifer R, Kaiser, Brent N, Sedgley, Margaret, Ford, Christopher M

The secondary metabolite amygdalin is a cyanogenic diglucoside that at high concentrations is associated with intense bitterness in seeds of the Rosaceae, including kernels of almond ('Prunus dulcis' (Mill.), syn. 'Prunus amygdalus' D.A. Webb Batsch). Amydalin is a glucoside of prunasin, itself a glucosider of 'R'-mandelonitrile (a cyanohydrin). Here we report the isolation of an almond enzyme (UGT85A19) that stereo-selectively gucosylates 'R'-mandelonitrile to produce prunasin. In a survey of developing kernels from seven bitter and 11 non-bitter genotypes with polyclonal antibody raised to UGT85A19, the enzyme was found to accumulate to higher levels in the bitter types in later development. This differential accumulation of UGT85A19 is associated with more than three-fold greater mandelonitrile glucosyltrajsferase activity in bitter kernels compared with non-bitter types, and transcriptional regulation was demonstrated using quantitative-PCR analysis. UGT85A19 and its encoding transcript were most concentrated in the testa (seed coat) of the kernel compared with the embryo, and prunasin and amygdalin were differentially compartmentalised in these tissues. Prunasin was confined to the testa and amygdalin was confined to the embryo. These results are consisted with the seed coat being an important site of synthesis of prunasin as a precursor of amygdalin accumulation in the kernel. The presence of UGT85A19 in the kernel and other tissues of both bitter and non-bitter types indicates that its expression is unlikely to be a control point for amygdalin accumulation and suggests additional roles for the enzyme in almond metabolism.

2013, McFadyen, Lisa Maree, Robertson, David, Sedgley, Margaret, Kristiansen, Paul, Olesen, Trevor D

Yields of macadamia ('Macadamia integrifolia', 'M. tetraphylla', and hybrids) orchards tend to increase with increasing tree size up to ≈94% light interception. Beyond this, there is some indication that crowding leads to yield decline, but the evidence is limited to one site. Increasing tree size and orchard crowding also present numerous management problems, including soil erosion, harvest delays, and increased pest and disease pressure. The aim of this study was to better characterize long-term yield trends in mature orchards and to assess the effects of manual and mechanical pruning strategies on yield, nut characteristics, tree size, and economics. We monitored yield at four sites in mature '344' and '246' orchards for up to seven years and confirmed a decline in yield with crowding for three of the sites. There was a small increase in yield over time at the fourth site, which may reflect the lower initial level of crowding and shorter monitoring period compared with the other sites, and highlights the need for long-term records to establish yield trends. Pruning to remove several large limbs from '246' trees to improve light penetration into the canopy increased yield relative to control trees but the effect was short-lived and not cost-effective. Removal of a codominant leader from '344' trees reduced yield by 21%. Annual side-hedging of '246' trees reduced yield by 12% and mechanical topping of '344' trees caused a substantial reduction in yield of up to 50%. Removal of limbs in the upper canopy to reduce the height of '344' trees had less effect on yield than topping but re-pruning was not practical because of the extensive regrowth around the pruning cuts. Tree size control is necessary for efficient orchard management, but in this study, pruning strategies that controlled tree size also reduced yield. Research into the physiological response to pruning in macadamia is required to improve outcomes.

2010, Rabiei, Gholamreza, Sedgley, Margaret, Gibson, John, Wu, Shubiao, Hunt, Peter

The almond, ['Prunus dulcis' (Mill) D.A. Webb syn. 'P. amygdalus' Batsch] is an important nut crop belonging to the family Rosaceae. Almond consumption has shown steady growth during the last decades due to the increased awareness of its excellent flavour, nutritional value and health benefits. The Australian climate provides great potential for growing almonds and the Australian almond breeding program was initiated in 1997 to develop cultivars better suited to growing conditions and to meet market demands. The construction of a genetic linkage map for one of the breeding populations (Nonpareil x Lauranne) was a part of this breeding program. This population comprised 181 individuals, of which 93 were used for map construction and quantitative trait loci (QTL) analysis in this study. Important traits that were investigated included flowering time, flower structures in relation to fruit set and autogamy and shell and kernel traits.

2012, McFadyen, Lisa Maree, Robertson, David, Sedgley, Margaret, Kristiansen, Paul, Olesen, Trevor David

Macadamia ('Macadamia integrifolia' Maiden and Betche, 'M. tetraphylla' Johnson and hybrids) orchards in Australia are typically hedged around anthesis (September). Such hedging reduces yields, largely through competition for carbohydrates between early fruit set and the post-pruning vegetative flush, but also through a reduction in photosynthetic capacity caused by the loss of canopy. We examined whether hedging at other times might mitigate yield losses. Hedging time was found to affect yields across four cultivars: 'A4', 'A38', '344' and '816'. Yield losses were lower for trees hedged in November-December than for trees hedged in September. Yields for trees hedged in June were higher than for trees hedged in September in one experiment, but were similar in a second experiment. Yield losses for September and October hedging were similar. Hedging time changed the pattern of fluctuations in stem water-soluble carbohydrates (WSC). WSC declined shortly after hedging in September, October or November, and the declines preceded increases in fruit abscission relative to unpruned control trees. The increase in fruit abscission was less pronounced for the trees hedged in November, consistent with the idea that fruit become less sensitive to carbon limitation as they mature.

2010, Wu, Shubiao, Franks, Tricia, Hunt, Peter, Wirthensohn, Michelle, Gibson, John, Sedgley, Margaret

Developed recently, high resolution melting (HRM) analysis is an efficient, accurate and inexpensive method for distinguishing DNA polymorphisms. HRM has been used to identify mutations in human genes, and to detect SNPs, INDELs and microsatellites in plants. However, its capacity to discriminate DNA variants in the context of complex haplotypes involving INDEL as well as SNP variants has not been examined until now. In this study, we genotyped an almond (Prunus dulcis (Mill.) D. A. Webb, syn. Prunus amygdalus Batsch) pseudotestcross mapping population that showed segregation of complex haplotypes associated with CYP79D16 promoter sequence. The 175 bp region in question included a 7 bp INDEL and 3 SNPs, and manifested as three different haplotypes in the parents. Thus, with one homozygous and one heterozygous parent, two relevant genotypes were identified in the mapping population. Although the population displayed monomorphism with respect to the INDEL and one of the SNPs, HRM was sufficiently sensitive to distinguish genotypes on the basis of the two informative SNPs, and the resulting data were used to map CYP79D16 to linkage group 6 of the almond genome. Thus the capacity of HRM to resolve genotypes arising from complex haplotypes has been demonstrated, and this has important implications for the design of efficient HRM markers for various genetic applications including mapping, population studies and biodiversity analyses.

2004, Wu, S, Collins, G, Sedgley, M

An integrated molecular linkage map of olive (Olea europaea L.) was constructed based on randomly amplified polymorphic DNA (RAPD), sequence characterized amplified region (SCAR), and microsatellite markers using the pseudo-testcross strategy. A mapping population of 104 individuals was generated from an F1 full-sib family of a crossbetween 'Frantoio' and 'Kalamata'. The hybridity of the mapping population was confirmed by genetic similarity and nonmetric multidimensional scaling. Twenty-three linkage groups were mapped for 'Kalamata', covering 759 cM of the genome with 89 loci and an average distance between loci of 11.5 cM. Twenty-seven linkage groups were mapped for 'Frantoio', covering 798 cM of the genome with 92 loci and an average distance between loci of 12.3 cM. For the integrated map, 15 linkage groups covered 879 cM of the genome with 101 loci and an average distance between loci of 10.2 cM. The size of the genomic DNA was estimated to be around 3000 cM. A sequence characterized amplified region marker linked to olive peacock disease resistance was mapped to linkage group 2 of the integrated map. Thesemaps will be the starting point for studies on the structure, evolution, and function of the olive genome. When the mapping progeny pass through their juvenile phase and assume their adult characters, mapping morphological markers and identification of quantitative trait loci for adaptive traits will be the primary targets.

2008, Longbottom, ML, Dry, PR, Sedgley, Margaret

Background and Aims: Following our original discovery of star flowers on grapevines in Australia, further variants of the star flower phenotype have been discovered on other varieties in South Australia. The objective of this report was to describe star flowers on 'Vitis vinifera' L. cvs Chardonnay and Shiraz. Methods and Results: Field and microscopic observations revealed that star flowers on both varieties opened precociously; there was neither pollen nor pollen tubes on the stigmas of the star flowers, and both Chardonnay and Shiraz star flowers contained aberrant ovules. The Chardonnay vines with star flowers produced bunches with an abundance of seedless berries; however, star flowers on Shiraz vines did not develop into berries. Conclusions: The inability of the Shiraz star flowers to develop into berries suggests that either there are two different mutations affecting these two varieties, or else the difference lies in their contrasting parthenocarpic tendencies. Significance of the Study: The star flower variants described here and previously may be the result of deviations to the normal molecular pathway for flower development in 'Vitis' species. The identification of star flowers on numerous varieties and in several different regions suggests that the occurrence of star flowers may be more widespread than previously realised, and the association of star flowers with the production of seedless berries and poor fruitset suggests that star flowers may also play a significant role in the problem of poor fruitset.

2008, Seifi, Esmaeil, Guerin, Jenny, Kaiser, Brent, Sedgley, Margaret

The influence of flower position on the inflorescence on opening day, gender, and petal persistence was studied in three olive cultivars: Manzanillo, Mission, and Frantoio. In each cultivar, 45 inflorescences were checked every morning from flower opening to petal fall. Perfect flowers opened mainly in the beginning of the flower opening period, and staminate flowers opened later. Flower position on the inflorescence had a highly significant effect on the opening day in all cultivars. Terminal flowers and the flowers located on the primary branches opened earlier than the flowers located on the secondary branches. Flower position had also a highly significant effect on gender in Manzanillo and Mission. In Manzanillo, the secondary branches had fewer perfect flowers than the primary branches. In Mission, the secondary branches had no perfect flowers at all. Among the primary branches, the branch arising immediately next to the terminal flower had the latest flowers to open and the lowest percent of perfect flowers. In Manzanillo, perfect flowers had significantly longer petal persistence than staminate flowers. To study flower competition within the inflorescence, the distal half of 120 inflorescences, on which the flowers tend to be perfect, in three trees of Manzanillo were removed about 1 month before full bloom. There was a highly significant effect on the percent of perfect flowers that opened on the proximal half. Flower competition may be a reason for pistil abortion in flowers located on secondary branches.