Sunlight intensity and UV radiation may affect free-range hens' use of the outside range, particularly when sunlight is intense with a high UV index. However, it is uncertain what aspect of sunlight (brightness or UV) may be most aversive to hens to discourage them from leaving standard indoor lighting conditions to venture outdoors. A controlled indoor-based choice study was conducted to determine whether hens showed preferences for different light wavelengths and intensities that may affect outdoor range usage. Cage-reared ISA Brown laying hens (n = 84) at 44 wk of age in 3 groups (28 hens/group) were tested for preferences of indoor standard light emitting diode (LED) white light (control) vs. one of three different treatment lights: 1) visible spectrum plus infrared wavelengths (VIS); 2) visible spectrum plus UVA wavelengths (UVA); and (iii) visible spectrum plus UVA and UVB wavelengths (UVA/B) presented successively at low, medium, or high levels of intensity. Hens within each group were individually tested for 2 h in an apparatus with 2 compartments (control vs. treatment) connected by a tunnel on both sides. Videos of hens' time spent in each compartment and behaviors were decoded and analyzed using GLMM. Hens spent more time under the low intensity of the UVA/B light treatment (62%), the low intensity of VIS light (61%), medium intensities of both UVA/B light (60%), and UVA light (59%), and the high intensity of the VIS light (58%) when compared with control light (all P ≤ 0.05). Hens spent less time feeding under all intensities of UVA light (all P ≤ 0.03) and showed more foraging, ground pecking, and preening at lower levels of UVA/B light (P < 0.05). The study suggests that UVA/B light (sunlight) may have positive effects for hen range use, but during peak sun intensities, hens may need additional measures (e.g., shelter) to protect themselves. Confirmation of these findings in a free-range setting is needed.

1. The objective of this study was to investigate the association of using a multi-tier aviary system and access to range on flock uniformity in free-range laying hens, and to determine whether the extent of range use or flock uniformity can be predicted from the use of different levels of the aviary system.
2. A total of 13,716 Lohmann Brown hens from five commercial free-range flocks housed in identical houses on the same farm were individually weighed at 16 weeks of age and allocated to five replicate areas within each house. Hen movement in the multi-tier aviary system and on the range was individually monitored using radio frequency identification (RFID). All hens had access to the range from 18 to 22 weeks of age and were exposed to the same management conditions.
3. Whilst only one flock significantly changed its flock uniformity with time, they differed from each other in uniformity and body weight (P = 0.001).
4. Hens spent most of their available time on the lower aviary tier (7.29 ± 0.029 h/hen/day) and on the upper aviary tier (4.29 ± 0.024 h/hen/day) while the least amount of time was spent on the range and in the nest boxes (0.93 ± 0.005 h/hen/day and 1.48 ± 0.007 h, respectively, P = 0.001).
5. Range use was negatively correlated (r = −0.30) to the time spent on the upper aviary tier and positively correlated (r = 0.46) to the time spent on the lower aviary tier (P = 0.001). Bivariate analysis revealed that range and upper aviary resp. lower aviary tier usage had a significant curvilinear association.
6. In conclusion, the study showed that range use was associated to the time hens spent on the different tiers of the aviary system. Flock uniformity varied between flocks but was not associated with either range and aviary system usage.