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Clay, Jonathon
- PublicationValidation of automatic systems for monitoring the licking behaviour in Angus and Brahman cattle(Elsevier BV, 2022-02)
; ; ; ; ; ; Monitoring feeding time in ruminants is one means to quantify feed intake. In grazing cattle offered feed supplement blocks, time spent licking can provide valuable information in estimating the level of blocks being ingested. This current study aimed to 1) assess an ear-tag accelerometer's capability to identify the licking behaviour at supplement blocks in grazing cattle and 2) evaluate the performance of the ear-tag accelerometer and radio-frequency identification (RFID) system to predict the individual time spent licking. Two breed groups of Angus (n = 7) and Brahman (n = 7) beef heifers were kept in two separate yards over 28 days. Each heifer was fitted with an ear-tag containing a tri-axial accelerometer set at 12.5 Hz frequency. Feed supplement blocks were provided through an RFID-equipped automatic supplement weighing unit within each yard, with access to the unit being given daily only from 16:00 h - 20:00 h. The accelerometer classification model developed using support vector machine (SVM) algorithm could distinguish between licking and non-licking behaviours, with an accuracy, sensitivity, F1 score, Cohen's kappa coefficient, and Matthew's correlation coefficient (MCC) of 86%, 93%, 0.88, 0.70, and 0.77 for Angus and 87%, 93%, 0.89, 0.73, and 0.79 for Brahman heifers. Time spent licking predicted by accelerometers were acceptable with a mean absolute error (MAE) of 22% and 11%, modelling efficiency (MEF) of 0.81 and 0.94, concordance correlation coefficient (CCC) of 0.88 and 0.96, and a ratio of root mean square prediction error (RSR) of 0.44 and 0.25, for Angus and Brahman heifers, respectively. However, the RFID system derived predictions of time spent licking in grazing heifers were unacceptable for both breeds. Overall, the ear-tag accelerometer offers the potential to predict individual time spent licking in grazing cattle to estimate block supplement intake.
- PublicationThe effects of dietary nitrate on plasma glucose and insulin sensitivity in sheep(Wiley-Blackwell Verlag GmbH, 2019-11)
; ; ; ; ; ; Nitrate (NO₃¯) is an effective non‐protein nitrogen source for gut microbes and reduces enteric methane (CH₄) production in ruminants. Nitrate is reduced to ammonia by rumen bacteria with nitrite (NO₂¯) produced as an intermediate. The absorption of NO₂¯ can cause methaemoglobinaemia in ruminants. Metabolism of NO₃¯ and NO₂¯ in blood and animal tissues forms nitric oxide (NO) which has profound physiological effects in ruminants and has been shown to increase glucose uptake and insulin secretion in rodents and humans. We hypothesized that absorption of small quantities of NO₂¯ resulting from a low‐risk dose of dietary NO₃¯ will increase insulin sensitivity (SI) and glucose uptake in sheep. We evaluated the effect of feeding sheep with a diet supplemented with 18 g NO₃¯/kg DM or urea (Ur) isonitrogenously to NO₃¯, on insulin and glucose dynamics. A glucose tolerance test using an intravenous bolus of 1 ml/kg LW of 24% (w/v) glucose was conducted in twenty sheep, with 10 sheep receiving 1.8% supplementary NO₃¯ and 10 receiving supplementary urea isonitrogenously to NO₃¯. The MINMOD model used plasma glucose and insulin concentrations to estimate basal plasma insulin (Ib) and basal glucose concentration (Gb), insulin sensitivity (SI), glucose effectiveness (SG), acute insulin response (AIRg) and disposition index (DI). Nitrate supplementation had no effect on Ib (p > .05). The decrease in blood glucose occurred at the same rate in both dietary treatments (SG; p = .60), and there was no effect of NO³¯ on either Gb, SI, AIRg or DI. This experiment found that the insulin dynamics assessed using the MINMOD model were not affected by NO₃¯ administered to fasted sheep at a low dose of 1.8% NO₃¯ in the diet. - PublicationVitamin D Metabolite Effects on Mineral Dynamics and Bone Physiology in Ruminants - Data(University of New England, 2023-01-19)
; ; ; ; This dataset was created during the project 'Vitamin D Metabolite Effects on Mineral Dynamics and Bone Physiology in Ruminants'. It consists of Bone Mineral Density Data, ICP Mineral Retention Data and Plasma Data extracted from CT scans of sheep conducted in Armidale during 2021. The data is organised into individual folders for each day of scans - PublicationDietary nitrate and presence of protozoa increase nitrate and nitrite reduction in the rumen of sheep(Wiley-Blackwell Verlag GmbH, 2020-09)
; ; ; ; ; Nitrate (NO3–) supplementation is an effective methane (CH4) mitigation strategy for ruminants but may produce nitrite (NO2–) toxicity. It has been reported that rumen protozoa have greater ability for NO3– and NO2– reduction than bacteria. It was hypothesised that the absence of ruminal protozoa in sheep may lead to higher NO2– accumulation in the rumen and a higher blood methaemoglobin (MetHb) concentration. An in vivo experiment was conducted with defaunated (DEF) and faunated (FAU) sheep supplemented with 1.8% NO3– in DM. The effects of rumen protozoa on concentrations of plasma and ruminal NO3– and NO2–, blood MetHb, ruminal volatile fatty acid (VFA) and ruminal ammonia (NH3) were investigated. Subsequently, two in vitro experiments were conducted to determine the contribution of protozoa to NO3– and NO2– reduction rates in DEF and FAU whole rumen digesta (WRD) and its liquid (LIQ) and solid (SOL) fractions, incubated alone (CON), with the addition of NO3– or with the addition of NO2–. The results from the in vivo experiment showed no differences in total VFA concentrations, although ruminal NH3 was greater (p < .01) in FAU sheep. Ruminal NO3–, NO2– and plasma NO2– concentrations tended to increase (p < .10) 1.5 hr after feeding in FAU relative to DEF sheep. In vitro results showed that NO3– reduction to NH3 was stimulated (p < .01) by incoming NO3– in both DEF and FAU relative to CON digesta. However, adding NO3– increased (p < .05) the rate of NO2– accumulation in the SOL fraction of DEF relative to both fractions of FAU digesta. Results observed in vivo and in vitro suggest that NO3– and NO2– are more rapidly metabolised in the presence of rumen protozoa. Defaunated sheep may have an increased risk of NO2– poisoning due to NO2– accumulation in the rumen. - PublicationDietary 1α-hydroxyvitamin D3 increases the concentration of phosphorus in the plasma of ewes(Australian Association of Animal Sciences, 2022-07-08)
; ; ; ; ; ; Vitamin D metabolites provide a novel method to increase the efficiency of bodily phosphorus (P) and calcium (Ca) use by increasing absorption and retention along with inducing bone remodelling and deposition. These compounds may have potential application for livestock where P demand is high and in grazing environments that are deficient in P. The non-endogenous compound 1α-hydroxyvitamin D3 (1(OH)D3) utilised in the treatment of human osteoporosis bypasses the tightly regulated step of 1α-hydroxylase in the kidney to create the active hormone form of the vitamin, 1,25α dihydroxyvitamin D3. The few available ruminant studies have shown that it has a positive effect on blood P and Ca status but studies have been restricted to intramuscular injection over a relatively short period of time (Sachs et al. 1987; Naito et al. 1987; Braithwaite 1980). It was hypothesised that dietary 1(OH)D3 supplementation would have a positive and sustained effect on plasma phosphorus concentrations over an 84-day period, utilising sheep as a model ruminant.