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McMillan, Mary
- PublicationComparing different EEG connectivity methods in young males with ASD(Elsevier BV, 2020-04-06)
; ; ; ; Although EEG connectivity data are often used to build models of the association between overt behavioural signs of Autism Spectrum Disorder (ASD) and underlying brain connectivity indices, use of a large number of possible connectivity methods across studies has produced a fairly inconsistent set of results regarding this association. To explore the level of agreement between results from five commonly-used EEG connectivity models (i.e., Coherence, Weighted Phased Lag Index- Debiased, Phase Locking Value, Phase Slope Index, Granger Causality), a sample of 41 young males with ASD provided EEG data under eyes-opened and eyes-closed conditions. There were relatively few statistically significant and/or meaningful correlations between the results obtained from the five connectivity methods, arguing for a re-estimation of the methodology used in such studies so that specific connectivity methods may be matched to particular research questions regarding the links between neural connectivity and overt behaviour within this population. - PublicationSensory Features and Bi-directional EEG Connectivity in Young Autistic Males(Springer New York LLC, 2022-04)
; ; ; ; Several lines of research suggest that autism is a neurological phenomenon, but the precise associations between neurological activity and the key diagnostic symptoms of autism are yet to be completely clarified. This study examined EEG connectivity and Sensory Features (SF) in a sample of young autistic males by examining bi-directional neural connectivity between separate brain regions as the key potential correlate of SF. Forty male autistic participants aged between 6 and 17 years, with an IQ of at least 70, underwent EEG measurements of their Frontal, Occipital and Temporal region responses to low-, medium-, and high-intensity audiovisual stimulus conditions. EEG connectivity data were analysed via Granger Causality. SF was measured via parent responses about their sons on the Child Sensory Profile (2nd ed.) (CSP-2). There were significant (p < .05) correlations between right hemisphere Frontal and Temporal connectivity and CSP-2 dominant scores, largely due to lower Temporal-to-Frontal than Frontal-to-Temporal connectivity. There were no significant correlations between general CSP-2 scores and EEG connectivity data collected during audiovisual stimuli. These results confirm and extend previous findings by adding bi-directional connectivity as an index of brain activity to other studies that used only uni-directional connectivity data when measuring SF. Although there may be a discrepancy between the kinds of information collected via instruments such as the CSP-2 and actual brain electrical connectivity across major regions, these results hold implications for the use of brain-training interventions with autistic boys.
- PublicationGirls' cortisol concentrations, mothers' anxiety, and self- versus parent-ratings of autistic girls' anxiety(Elsevier Inc, 2021-03)
; ; ;Mandy, Will; Background: Autistic girls’ change in salivary cortisol concentrations from morning to afternoon were compared with their parents’ own anxiety states as potential correlates of the autistic girls’ self-ratings of their anxiety and those given by their parents about their daughters’ anxiety.
Methods: Thirty-three autistic girls (6 yr to 10 yr, M = 8.21 yr, SD = 1.29 yr) and 19 autistic adolescent females (11 yr to 15 yr, M = 12.74 yr, SD = 1.52 yr) completed anxiety inventories about themselves and provided saliva samples in the morning and afternoon. Their mothers also rated their daughters’ anxiety and their own anxiety.
Results: There were no significant differences between mothers’ and daughters’ ratings of the latter’s GAD. The autistic girls’ self-ratings of their anxiety were more strongly influenced by their mothers’ anxiety in the younger girls, but more responsive to their own chronic stress state (i.e., cortisol) in the adolescent girls.
Conclusions: Mothers’ evaluations of their autistic daughters’ anxiety appear to be strongly influenced by their own anxiety. There was also some indication that autistic girls may disguise their anxiety.
- PublicationResearch Ready: a student-initiated workshop model for developing foundational research skills(American Society for Microbiology, 2023-12)
;Sivarajah, Nivetha ;Irranious, Jenevan A ;Krishnamoorthy, Sivagini ;Kalaineethan, Thayaparan ;Kugathasan, Deluxeani ;Sivanantham, Uventhikka; Travel restrictions, pandemics, economic downturn, and increasing costs in organizing workshops all impact on face-to-face training of undergraduates planning to undertake research. The inability to obtain basic, first-hand information regarding research in practice causes undue stress for students and leads to unrealistic expectations regarding research projects. Here, we describe how a student initiated online workshop, co-designed by a group of undergraduate leaders in conjunction with a panel of international academic researchers, and enabled the delivery of an introductory workshop on research training to meet student needs. Post-workshop, over 80%–95% of the participants rated their understanding of different aspects of research in practice as either being good or excellent. The design of this workshop provides an innovative template, in particular for resource-restricted countries, on how student-initiated workshops with multi-institutional academic collaboration could enhance training in research practice.
- PublicationDoes the cortisol: CRP ratio inform the measurement of individual burden of illness for depression in community samples?(Elsevier BV, 2021-01)
; ; ; ; Background: Individual Burden of Illness for Depression (IBI-D) represents a method for assessing the degree to which depressive symptomatology reduces functioning. Although the IBI-D has been shown to provide more information than measures of depressive symptomatology alone, previous studies have relied upon participant self-reports, and have not included an objective measure of physiological functioning. Such an index might add to the validity and informational value of IBI-D data.
Methods: Responses to scales measuring depressive symptoms, functional impairment due to mental and physical health, and saliva samples were collected from 111 community volunteers from rural Australia. Saliva was assayed for cortisol and C-Reactive Protein concentrations, and the ratio of these was used as an indicator of the balance between the Hypothalamic-Pituitary-Adrenal axis and the inflammatory response from the immune system.
Results: Principal Components Analysis (PCA) produced two- and three-factor solutions from the psychological and biological data, and were used to form weighted models of two IBI-D equations. Stepwise regression analysis indicated that the addition of the biological index to the second IBI-D made a significant extra contribution to variance in depression score.
Limitations: No longitudinal data were collected, participants were from a single geo-cultural region, and were self-selecting. Clinician interviews might augment participant self-reports.
Conclusions: Valid assessment of the self-reported symptoms of depression provides one aspect of diagnostic information, but the addition of biological information can further inform clinicians and researchers about the effect that these symptoms have upon individual patient functioning.
- PublicationInvestigation of molecular and cellular aspects of cotton black root rot disease and the potential for biological control-Dataset(University of New England, 2021-03-11)
;Abd Oun, Hamid; ; The dataset includes four sets of data:
1. Data of chapter 2: abundance of TB gene in five farms, the soil physio-chemical parameters of these farms, the correlation of each the abundance of gene with soil physio-chemical parameters for each farm ( farm B, C , P3 , Q and T).
2. Data of chapter 3: include the data of the effect of temperature on the virulence and pathogen loading in soil at 20 and 25C, percentage of lesion, shoot water content at 20 and 25C, row data of gene copy number in soil, row data of gene copy number in root, and correlation of all parameters ( soil, root , lesion and water content).
3. Data of chapter 4: include the row data of the growth direction, dry weight SP7 and SP245, optical density of SP7 and SP245 and visual data.
4. Data of chapter 5: visual data including attach A. brasilence to border cell, bacterial binding assay, B.rouxiae with border cell and fungal interaction with border cell - PublicationBioaccessibility constrains the co-composting bioremediation of field aged PAH contaminated soils(Elsevier Ltd, 2020-04)
;Leech, Calvin; ; ; ; ; Co-composting for the remediation treatment of aged polycyclic aromatic hydrocarbons (PAHs) in field contaminated soil was examined in this study. Two PAH contaminated soils collected from a manufacturing gas plant site with high PAH concentrations (184.00 ± 3.99 mg kg⁻¹ and 490.0 ± 43.53 mg kg⁻¹) were co-composted with cattle manure and wheat straw for up to 56 days in laboratory microcosms. A range of optimised co-composting treatments including addition of Triton-X100 surfactant and a biological surfactant from the Australian Red Ash tree (Alphitonia excelsa) were tested. There was no significant decrease in the PAH concentrations in any of the different co-composting treatments for either of the soils, despite an 87% decrease in a phenanthrene spike (100 mg kg⁻¹), and evidence of PAH microbial catabolic activity. Non-exhaustive extraction using hydroxypropl-β-cyclodextrin (HP-β-CD) and linear regression modelling confirmed that PAH bioaccessibility constrained PAH co-composting remediation in these soils. The results demonstrate that co-composting alone is not an effective remediation strategy for certain aged PAH contaminated soils. For these soils some form of pre-composting chemical or engineering treatment is required to overcome bioaccessibility limitations in successful remediation. - PublicationThe physical and mental health effects of housing homeless people: A systematic review(Wiley-Blackwell Publishing Ltd, 2022-02)
;Onapa, Hebaat; ; ; ;MacLure, Katie ;Smith, LeeHousing is a significant determinant of health and is widely accepted as a key solution to address some of the health disparities that exist among the homeless. It is estimated that 150 million people worldwide are homeless, and approximately 1.8 billion lack adequate housing. However, understanding of how housing has a positive impact on the health of the homeless remains unclear and underdeveloped. This systematic review investigates intervention studies that report on the physical and mental health effects of housing homeless persons. A search of PubMed, PsycINFO, EBSCOHost-Academic Search Complete and the Cochrane Library was conducted for peer-reviewed articles published in English from 1999 to 2020 that had a combination of at least one housing intervention and health outcome, with a homeless sample. Three previous reviews and 24 studies were included for analysis. Most of the studies (n = 20) encompassed permanent supportive housing interventions that emphasised placing homeless people with mental illness directly into affordable housing with access to support services. The primary health outcomes reported were general physical and mental health, well-being, and quality of life. Despite inconsistent findings and significant issues identified in the reviewed literature, housing (in the short term) improves some aspects of health in homeless populations with human immunodeficiency virus, anxiety and depression.
- PublicationAuxin Biosynthesis, Signalling and Function During Early Stages of Endosperm Development in Rice (Oryza sativa L.)(University of New England, 2021-02-23)
; ; ; Rice endosperm feeds more than half of the world's population. Its development is a complex process with multiple layers of regulation. The molecular events occurring during its early stages of development determine largely the final grain size and weight, which are key components of grain yield. A large and rapid increase in the content of indole-3-acetic acid (IAA), the main in planta auxin, occurs during early endosperm development. However, auxin action in early endosperm is poorly understood. In this study, I aimed to explore spatio-temporal expression of IAA biosynthesis and signalling genes during early stages of endosperm development in rice. My other aim was to assess the effects of IAA on grain fill and to determine the auxin-response genes in early grains. I found that OsYUC12, one of the three grain-specific OsYUCCAs, was expressed in the aleurone, sub-aleurone and embryo at 3-8 days after pollination (DAP), suggesting early aleurone, sub-aleurone and embryo as sites of IAA biosynthesis. The non-canonical OsIAA29 was strongly co-expressed with OsYUC12. Its spatial expression was restricted to dorsal aleurone, where it could be part of an auxin signalling pathway. OsIAA29 has orthologues only in cereal and non-cereal grass species; expression of the orthologues is early grain- or endosperm-specific. This gene may have evolved independently in the grass family (Poaceae) and acquired conserved functions related to aleurone development. Furthermore, a cluster of MYB transcription factors orthologous to maize Myb-Related Protein-1 (ZmMRP-1) may act downstream of the auxin signalling in dorsal aleurone and regulate its differentiation in relation to apoplastic nutrient transfer. Taking my cue from IAA biosynthesis and signalling, I used a chemical approach to determine its function in early rice grains. Application of two IAA biosynthesis inhibitors, Lkynurenine and 4-phenoxyphenylboronic acid (PPBo), to rice spikelets from 3 to 10 DAP reduced significantly grain IAA levels. Genes encoding small cysteine-rich peptides, seed storage proteins and amino acid transporters were down-regulated in auxin-deficient grains at 5 DAP. These genes are expressed specifically in the aleurone, sub-aleurone and embryo. Auxin may also regulate homeostasis of ethylene, cytokinins, gibberellins and abscisic acid in early grains. Inhibitor-treated panicles showed extensive post-fertilisation seed abortion, which consequently led to significant reduction in their total weight at maturity. This suggested a crucial role of auxin in grain retention, probably through regulation of coenocyte cellularisation. Furthermore, I showed short-lived expression of three OsARFs (OsARF13, OsARF14 and OsARF16) and two OsAUX/IAAs (OsIAA8 and OsIAA14) in early grains at 2-6 DAP. OsARF16 is most likely to interact with OsIAA8 and OsIAA14 in early endosperm. Thus, the results from this study suggested auxin action during key events of early endosperm development such as cellularisation of coenocyte nuclei, early aleurone differentiation and initiation of starch and storage protein biosynthesis, which take place at 3-7 DAP. These findings will point towards new areas of research that will further our understanding of the role of auxin in cereal endosperm development. - PublicationMolecular assessment of soil microbial function and community response under differing soil management practices(University of New England, 2020-11)
;Aldorri, Sind Shamel Omer; ; This dataset is divided in to three parts:
part 1: Chapter 3 and 4: include the functional genes of N cycle (amoA-B, amoA-arch and ureCgenes ), C cycle ( mcrA pmoA genes), and S cycle (aprA gene). These data were obtained by qPCR. Additional to the total DNA and 16SrRNA and the physiological parameter of soil ( OC, P,Fe, Cu, Mn, Zn ,NH4 and NO3). there is a sheet for all graphs of genes.
part 2: chapter 5: include the row data of Biology Eco plate and NGS. For Biology Eco plate, I include Richness, Eveness, SAWCD, AWCD, and row data of farm B, C, P3, Q and T at 72hr. It is also include the the five guilds of Biolog eco-plate (carbohydrates, polymers, amino acid, carboxylic and acetic acid and amines/amides). For NGS, I include OTU graph, faith-pd, PcoA plot, phylogenetic bars, weight-Unifrac and unweight-Unifrac.
part 3: include the data that were performed in the glasshouse, which include the functional genes that amplified by qPCR (amoA-B, amoA-arch, ureC,nirK,nosZ,nirS pmoA, mcrA and aprA genes. the other data belong to this chapter is a table of physicochemical parameter of soil.