Terrestrial laser scanning (TLS) data provide 3-D measurements of vegetation structure and have the potential to support the calibration and validation of satellite and airborne sensors. The increasing range of different commercial and scientific TLS instruments holds challenges for data and instrument interoperability. Using data from various TLS sources will be critical to upscale study areas or compare data. In this paper, we provide a general framework to compare the interoperability of TLS instruments. We compare three TLS instruments that are the same make and model, the RIEGL VZ-400. We compare the range accuracy and evaluate the manufacturer's radiometric calibration for the uncalibrated return intensities. Our results show that the range accuracy between instruments is comparable and within the manufacturer's specifications. This means that the spatial XYZ data of different instruments can be combined into a single data set. Our findings demonstrate that radiometric calibration is instrument specific and needs to be carried out for each instrument individually before including reflectanceinformation in TLS analysis. We show that the residuals between the calibrated reflectance panels and the apparent reflectance measured by the instrument are greatest for highest reflectance panels (residuals ranging from 0.058 to 0.312).

Terrestrial laser scanning (TLS) can be used to characterize a woodland site by measuring structural attributes of the vegetation community. In Australia, government funded programs monitor vegetation structure using manual field surveys to assess change and ecological condition. In this study, we examined whether structural attributes commonly assessed in woodland ecology surveys can be extracted from a single TLS scan. Attributes of the ground, shrub and overstory vegetation layers were evaluated at nine open woodland sites in central Western Queensland. We used 0.1 m voxels to aggregate returns. Our results show that, compared with field assessment by highly experienced ecologists, TLS can rapidly characterize structural attributes for tree canopy cover, maximum tree height, average tree height (R² > 0.9) and average diameter at breast height (R² = 0.77). However, we could not accurately determine shrub height, shrub canopy cover, shrub average height, ground cover (grass, litter and coarse woody debris) or the number of trees per hectare (R² < 0.45). By analysing local minima in the histogram of the maximum height, we determined height thresholds for canopy strata, and applied these to determine the canopy layer with the most biomass – the ecologically dominant layer (EDL). While these results are promising for overstory assessment and defining canopy strata heights using TLS, they suggest that future research should focus on investigating improved classification methods to separate laser returns into shrub and tree objects for structural assessment at the plot scale.