Calcium oxalate (CaOx) crystals are a common natural feature of many plant families, including the Leguminosae. The functional role of crystals and the mechanisms that underlie their deposition remain largely unresolved. In several species, the seasonal deposition of crystals has been observed. To gain insight into the effects of rainfall on crystal formation, the morphology, distribution and accumulation of calcium oxalate crystals in phyllodes of the leguminous 'Acacia' sect. 'Juliflorae' (Benth.) C. Moore & Betche from four climate zones along an aridity gradient, was investigated. The shapes of crystals, which include rare Rosanoffian morphologies, were constant between species from different climate zones, implying that morphology was not affected by rainfall. The distribution and accumulation of CaOx crystals, however, did appear to be climate-related. Distribution was primarily governed by vein density, an architectural trait which has evolved in higher plants in response to increasing aridity. Furthermore, crystals were more abundant in acacias from low rainfall areas, and in phyllodes containing high concentrations of calcium, suggesting that both aridity and soil calcium levels play important roles in the precipitation of CaOx. As crystal formation appears to be calcium-induced, we propose that CaOx crystals in Acacia most likely function in bulk calcium regulation.

Success of establishing native trees in cool temperate environments depends on the ability of seedlings to withstand subzero temperatures and recurrent frosts. This study compared the survival and growth of five tree and shrub species with two guard types at three landscape positions in an upland pasture. Seedlings were planted between December 2013 and March 2014. Half of the seedlings were planted in tall Corflute® guards (60 cm high), and the remaining seedlings were interplanted in milk cartons (30 cm). Seedling survival and height were measured in November 2014. Hourly temperature readings were recorded between March and November 2014. Seedling height for all species was greater in tall guards than milk cartons at all landscape positions, possibly at least partly due to etiolation. However, seedlings in tall guards survived better than seedlings in milk cartons at mid- and upper-slope positions. Higher temperatures may have benefited seedling performance by prolonging the growing season as average maximum temperature was significantly higher inside tall guards than milk cartons and ambient conditions at all landscape positions. Average daily temperature was significantly higher in tall guards compared to milk cartons and ambient conditions at the upper-slope site only. There were no significant differences in average minimum temperature between guard types at all landscape positions.