The primary objectives of the current thesis were to develop an inexpensive, high-throughput method of quantifying NH3 in solution, and an in vitro method of quantifying NH3 production from manure that would (1) evaluate the optimal microchamber design to quantify NH3 production rates from manure; (2) quantify the effect of disturbance of manure on NH3 production rates; (3) succeed as an alternative method of testing NH3 production to reduce the need for, or better inform, large-scale animal experiments (4) successfully evaluate the effectiveness of mitigation techniques. This data represents NH3 production over time using the in-vitro method and colorimetric methods in this thesis.