<p>An experiment was conducted to evaluate the effects of varying AME concentrations and protease supplementation on broiler performance and jejunal and ileal nutrient digestibility from 1 to 35 d of age. Ross £ Ross 308 male broilers (n = 1,008) were equally distributed into 48 floor pens and offered 1 of 6 dietary treatments (8 replicate pens/treatment). Dietary treatments consisted of a factorial arrangement with AME concentration (low-, moderate-, or highAME) and supplemental protease (without or with) as the main factors. Birds and feed were weighed on 1, 15, 29, and 35 d of age to determine body BW, BW gain (<b>BWG</b>), feed intake (<b>FI</b>) and feed conversion ratio (<b>FCR</b>). At 15 and 29 d of age, jejunal and ileal digesta contents were collected to determine nutrient digestibility. From 1 to 15 d of age, broilers offered moderateAME diets (<i>P</i> < 0.05) had 6.7, 7.1, 4.8% higher BW, BWG, FI, respectively, and a 2.1% lower FCR compared with those offered low-AME diets. Likewise, protease increased (<i>P</i> < 0.05) BW and BWG by 4.3 and 4.7%, respectively, and decreased (<i>P</i> < 0.05) FCR by 3.4%, compared with those offered the diets without protease. From 1 to 29 d of age, broilers offered high-AME diets had 2.9% lower (<i>P</i> < 0.05) FCR compared with those offered low-AME diets. Protease increased (<i>P</i> < 0.05) BW, BWG, and FI by 3.1, 3.2, and 4.2%, respectively, compared with the unsupplemented diets. From 1 to 35 d of age, broilers receiving high-AME diets had 2.9% lower (<i>P</i> < 0.05) FCR compared with those offered lowAME diets. Protease increased (<i>P</i> < 0.05) FCR by 1.0% compared with those offered unsupplemented diets. Jejunal (15 and 29 d of age) and ileal (29 d of age) starch digestibility and jejunal nitrogen digestibility (29 d of age) were lower (<i>P</i> < 0.05) in broilers offered high-AME diets compared with those offered low-AME diets. Both AME concentration and supplemental protease independently affected broiler performance, with responses being most apparent during early growth phases whereas digestibility measures were mainly influenced by AME concentration.</p>