Increasing anthropogenic impacts on biodiversity has been a cause for concern in Australia in recent years. Areas that hold high levels of endemic species and also face exceptional threats of destruction have been described as biodiversity hotspots. Ecological research focused on biodiversity hotspots will provide a better understanding of the flora and fauna of these regions and thus inform conservation strategies. Consequently, it is important to understand where biodiversity hotspots are located and how well they have been researched in the past. However, the choice of ecological research sites may be influenced by a variety of factors such as proximity to research institutions. This study utilized a geographic information system to investigate the spatial distribution of ecological research field sites in Australia and its territorial waters, the hotspots of the field sites around research institutions and the proximity of ecological research field sites from the main campus of the research institutions. Furthermore, these hotspots of ecological research were linked to biodiversity hotspots to identify the regions that were commonly depicted in the ecological literature and to identify others that may need more attention. We demonstrated that hotspots of ecological research were concentrated around research institutions, with a large number of field sites being located between 0 km and 500 km from the nearest institution, especially along the eastern coast. This study highlighted areas that have been the focus of much ecological research as well as areas that need more attention from ecologists to add new knowledge to Australian ecological science.