In current best commercial practice, pre-harvest fruit load predictions of mango orchards are provided based on a manual count of fruit number on up to 5% of trees within each block. However, the variability in fruit number per tree (coefficient of variation, CV, from 27 to 93% across ten orchards) was demonstrated to be such that the best case commercial sampling practice was inadequate for reliable estimation (to an error of 54–82 fruit/tree, and percentage error, PE, of 10% at a probability of 0.95). These results highlight the need for alternative methods for estimation of orchard fruit load. Pre-harvest fruit load was estimated for a case study orchard of 469 trees using (i) count of a sample of trees, (ii) in-field machine vision and (iii) correlation to a tree spectral index estimated using high resolution satellite imagery. A count of 5% of trees (23) in the trial orchard resulted in a PE of 31% (error of 37 fruit/tree), with a count of 157 trees required to achieve a PE of 10% (error of 12 fruit/tree). Sampling effort to achieve a PE of 10% was decreased by only 10% by sampling from aspatial k-means tree classifications based on machine vision derived fruit counts of all trees. Clustering based on tree attributes of canopy volume and trunk circumference was not helpful in decreasing sampling effort as these attributes were poorly correlated to fruit load (R² =0.21 and 0.17, respectively). In-field multi-view machine vision-based estimation of fruit load per tree achieved a R²= 0.97 and a RMSE = 14.8 fruit/tree against harvest fruit count per tree for a set of 18 trees (average = 88; SD = 82 fruit/tree), using a faster region convolutional neural network trained the previous season. The relationship between WorldView-3 (WV3) satellite spectral reflectance characteristics of sampled trees and fruit number was characterised by a R² = 0.66 and a RMSE = 56.1 fruit/tree. For this orchard, for which the actual fruit harvest was 56,720 fruit, the estimate based on a manual count of 5% of trees was 47,955 fruit, while estimates based on 20 iterations of stratified sampling (of 5% of trees in each cycle) had variation (SD) of 9597. The machine vision method resulted in an estimate of 53,520 (SD = 1960) fruit and the remote sensing method, 51,944 (SD = 26,300) fruit for the orchard.