<p>This study investigated the hypothesis that high dietary calcium (<b>Ca</b>) would stimulate necrotic enteritis (<b>NE</b>) and reduce performance, gut health, and nutrient digestibility, and if increased, phytase would reduce NE. Ross 308 male broilers (n = 768) were randomly distributed to 8 treatments in a factorial arrangement. Factors were NE challenge (no or yes), phytase level (500 or 1,500 FTU/kg using 500 FTU/kg matrix values), and Ca level (0.6 or 1.0% starter, 0.5 or 0.9% grower, 0.4 or 0.8% finisher) with the same level of available P (0.40 S, 0.35 G, and 0.35 F). There were 48 pens, 16 birds per pen and 6 replications. Half of the birds were challenged with <i>Eimeria spp</i> on day 9 and 108 CFU per mL of <i>Clostridium perfringens</i> strain EHE-NE18 on day 14 and 15. Gain was higher in birds fed high phytase on day 14 (<i>P</i> < 0.01), day 21 (<i>P</i> < 0.01), day 28 (<i>P</i> < 0.01), and day 35 (<i>P</i> < 0.01). Birds fed high phytase had greater livability on day 21 (<i>P</i> < 0.01). Ca was more digestible in high-Ca diets on day 16, and an NE × Ca interaction (<i>P</i> < 0.05) showed this effect to be more pronounced in unchallenged than in challenged birds. A challenge × Ca interaction for apparent ileal digestibility (<b>AID</b>) of crude protein (<b>CP</b>) (<i>P</i> < 0.05) indicated lower AID of CP in challenged birds fed high Ca. The challenge decreased AID of Ca (<i>P</i> < 0.01). Ca level had no impact on <i>C. perfringens</i> count, but it decreased <i>Lactobacillus</i> (<i>P</i> < 0.05) and <i>Bifidobacteria</i> (<i>P</i> < 0.05) populations in the ceca. High dietary Ca decreased feed conversion ratio. Overall (42 D), the highest WG was observed in unchallenged birds fed high Ca and high phytase with the lowest WG observed in NE-challenged birds fed low Ca and low phytase. The results suggest that full matrix values for high doses of phytase may be appropriate during NE challenge.</p>